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Last updated: 2020/11/22
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Histone H3 (phospho S28) monoclonal antibody, clone D6 

  • Catalog # : MAB18914
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic phosphopeptide of Histone H3.
  • Immunogen:
  • A synthetic phosphopeptide corresponding to residues surrounding S28 of human Histone H3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Flow Cytometry
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (50% glycerol, 0.1% BSA and 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of HeLa cell lysate with Histone H3 (phospho S28) monoclonal antibody, clone D6 (Cat # MAB18914). Lane 1: treated or Lane 2: untreated synchronized in metaphase by treatment with 0.4 ug/mL of nocodazole for 6 hr then metaphase cells were isolated by mitotic shake-off.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells with Histone H3 (phospho S28) monoclonal antibody, clone D6 (Cat # MAB18914). Secondary antibody only negative control (blue) or untreated (red) or treated with nocodazole (green).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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