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Last updated: 2022/6/26

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H2AFX (phospho S139) monoclonal antibody, clone 10000 

  • Catalog # : MAB18899
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic phosphopeptide of human H2AFX.
  • Immunogen:
  • A synthetic phosphopeptide corresponding to residues surrounding S139 of human H2AFX.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Flow Cytometry
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (50% glycerol, 0.1% BSA and 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of HEK293T cell lysate with H2AFX (phospho S139) monoclonal antibody, clone 10000 (Cat # MAB18899). Lane 1: treated or Lane 2: untreated with UV.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of 293T cells with H2AFX (phospho S139) monoclonal antibody, clone 10000 (Cat # MAB18899). Secondary antibody only negative control (blue) or untreated (red) or treated with UV and PMA (green).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3014
  • Gene Name:
  • H2AFX
  • Gene Alias:
  • H2A.X,H2A/X,H2AX
  • Gene Description:
  • H2A histone family, member X
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq
  • Other Designations:
  • H2AX histone
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