GAL4 monoclonal antibody, clone 8C-1
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant GAL4.
Immunogen
Recombinant protein corresponding to full length yeast GAL4.
Host
Mouse
Theoretical MW (kDa)
106
Reactivity
Yeast
Specificity
This antibody recognizes a 106 KDa protein by Western blot in yeast cell extracts corresponding to amino acids 1-147 of the GAL4 DNA binding region.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.08% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — GAL4
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Publication Reference
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The DNA binding domains of the yeast Gal4 and human c-Jun transcription factors interact through the zinc-finger and bZIP motifs.
Sollerbrant K, Akusjärvi G, Linder S, Svensson C.
Nucleic Acids Research 1995 Feb; 23(4):588.
Application：WB-Re, Recombinant protein.
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GAL4 is regulated by a glucose-responsive functional domain.
Stone G, Sadowski I.
The EMBO Journal 1993 Apr; 12(4):1375.
Application：IP, Yeast, YT6 strain.
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An amino-terminal fragment of GAL4 binds DNA as a dimer.
Carey M, Kakidani H, Leatherwood J, Mostashari F, Ptashne M.
Journal of Molecular Biology 1989 Oct; 209(3):423.
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The DNA binding domains of the yeast Gal4 and human c-Jun transcription factors interact through the zinc-finger and bZIP motifs.
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