AKT1 (phospho T308) monoclonal antibody, clone G12
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of 293T cell lysate with AKT1 (phospho T308) monoclonal antibody, clone G12 (Cat # MAB18788). Lane 1: treated or Lane 2: untreated with 20% FBS.
Flow Cytometry
Flow cytometric analysis of K562 cells with AKT1 (phospho T308) monoclonal antibody, clone G12 (Cat # MAB18788). Secondary antibody only negative control (blue) or treated with LY294002 (red) or with IFNa + IL-4 + pervanadate (green).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human AKT1.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding T308 of human AKT1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.1% BSA and 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of 293T cell lysate with AKT1 (phospho T308) monoclonal antibody, clone G12 (Cat # MAB18788). Lane 1: treated or Lane 2: untreated with 20% FBS.Flow Cytometry
Flow cytometric analysis of K562 cells with AKT1 (phospho T308) monoclonal antibody, clone G12 (Cat # MAB18788). Secondary antibody only negative control (blue) or treated with LY294002 (red) or with IFNa + IL-4 + pervanadate (green). -
Gene Info — AKT1
Entrez GeneID
207Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactome
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Pathway
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Disease
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