His-Tag monoclonal antibody, clone 2C5.1
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Specification
Product Description
Mouse monoclonal antibody raised against His-Tag.
Immunogen
His-Tag.
Host
Mouse
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.08% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
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Publication Reference
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S. pombe expression vector with 6x(His) tag for protein purification and potential for ligation-independent cloning.
Hosfield T, Lu Q.
Biotechniques 1999 Jul; 27(1):58.
Application:WB-Tr, Human, Yeast, Mammalian cells, Yeast cells.
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Detection of histidine-tagged fusion proteins by using a high-specific mouse monoclonal anti-histidine tag antibody.
Zentgraf H, Frey M, Schwinn S, Tessmer C, Willemann B, Samstag Y, Velhagen I.
Nucleic Acids Research 1995 Aug; 23(16):3347.
Application:ELISA, IF, IP, WB-Re, Human, Recombinant proteins, SAOS-2 cells.
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High-level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein gD synthesized by baculovirus-infected insect cells.
Sisk WP, Bradley JD, Leipold RJ, Stoltzfus AM, Ponce de Leon M, Hilf M, Peng C, Cohen GH, Eisenberg RJ.
Journal of Virology 1994 Feb; 68(2):766.
Application:WB-Re, WB-Tr, Insect, N/A, Recombinant proteins.
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S. pombe expression vector with 6x(His) tag for protein purification and potential for ligation-independent cloning.
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