NAPA monoclonal antibody, clone 4E4
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant NAPA.
Immunogen
Recombinant protein corresponding to human NAPA.
Host
Mouse
Theoretical MW (kDa)
36
Reactivity
Human
Specificity
This antibody specifically recognizes alpha SNAP as a single band of ~36 KDa on western blot of rat kidney, rat brain and MDBK cells and do not recognize beta SNAP.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (0.5-5 ug/mL)
Immunoprecipitation (2-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM sodium phosphate buffer, 150 mM NaCl, pH 7.5 (50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunoprecipitation
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Gene Info — NAPA
Entrez GeneID
8775Gene Name
NAPA
Gene Alias
SNAPA
Gene Description
N-ethylmaleimide-sensitive factor attachment protein, alpha
Omim ID
603215Gene Ontology
HyperlinkGene Summary
The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37%, 60%, and 67% identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively. Platelets contain some of the same proteins, including NSF, p115/TAP, alpha-SNAP, gamma-SNAP, and the t-SNAREs syntaxin-2 and syntaxin-4, that are used in many vesicular transport processes in other cell types. Platelet exocytosis uses a molecular mechanism similar to that used by other secretory cells, such as neurons, although the proteins used by the platelet and their modes of regulation may be quite different. [provided by RefSeq
Other Designations
alpha-SNAP
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