DNM2 monoclonal antibody, clone 3F5F3
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human DNM2.
Immunogen
Recombinant protein corresponding to amino acids 520-744 of human DNM2.
Host
Mouse
Theoretical MW (kDa)
98
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Western Blot (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: DNM2-hIgGFc transfected HEK293 cell lysates with DNM2 monoclonal antibody, clone 3F5F3 (Cat # MAB17963).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with DNM2 monoclonal antibody, clone 3F5F3 (Cat # MAB17963).Flow Cytometry
Flow cytometric analysis of HeLa cells with DNM2 monoclonal antibody, clone 3F5F3 (Cat # MAB17963) (Green). Red: Negative Control. -
Gene Info — DNM2
Entrez GeneID
1785Protein Accession#
P50570Gene Name
DNM2
Gene Alias
CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII
Gene Description
dynamin 2
Gene Ontology
HyperlinkGene Summary
Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Four alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq
Other Designations
dynamin II
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Interactome
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Pathway
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Disease
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