BCL2L2 monoclonal antibody, clone 4G12E6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: BCL2L2-hIgGFc transfected HEK293 cell lysates with BCL2L2 monoclonal antibody, clone 4G12E6 (Cat # MAB17942).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cervix cancer with BCL2L2 monoclonal antibody, clone 4G12E6 (Cat # MAB17942).
Immunofluorescence
Immunofluorescent staining of HeLa cells with BCL2L2 monoclonal antibody, clone 4G12E6 (Cat # MAB17942) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with BCL2L2 monoclonal antibody, clone 4G12E6 (Cat # MAB17942).
Flow Cytometry
Flow cytometric analysis of HeLa cells with BCL2L2 monoclonal antibody, clone 4G12E6 (Cat # MAB17942) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human BCL2L2.
Immunogen
Recombinant protein corresponding to amino acids 6-118 of human BCL2L2.
Host
Mouse
Theoretical MW (kDa)
20.7
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunofluorescence (1:200-1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: BCL2L2-hIgGFc transfected HEK293 cell lysates with BCL2L2 monoclonal antibody, clone 4G12E6 (Cat # MAB17942).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cervix cancer with BCL2L2 monoclonal antibody, clone 4G12E6 (Cat # MAB17942).Immunofluorescence
Immunofluorescent staining of HeLa cells with BCL2L2 monoclonal antibody, clone 4G12E6 (Cat # MAB17942) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis with BCL2L2 monoclonal antibody, clone 4G12E6 (Cat # MAB17942).Flow Cytometry
Flow cytometric analysis of HeLa cells with BCL2L2 monoclonal antibody, clone 4G12E6 (Cat # MAB17942) (Green). Red: Negative Control. -
Gene Info — BCL2L2
Entrez GeneID
599Protein Accession#
Q92843Gene Name
BCL2L2
Gene Alias
BCL-W, BCLW, KIAA0271
Gene Description
BCL2-like 2
Omim ID
601931Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. [provided by RefSeq
Other Designations
BCL2-like 2 protein|apoptosis regulator BCL-W
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Interactome
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Disease
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