ATG3 monoclonal antibody, clone 2C10A12
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: ATG3-hIgGFc transfected HEK293 cell lysates with ATG3 monoclonal antibody, clone 2C10A12 (Cat # MAB17913).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach carcinoma with ATG3 monoclonal antibody, clone 2C10A12 (Cat # MAB17913).
Immunofluorescence
Immunofluorescent staining of SMMC-7721 cells with ATG3 monoclonal antibody, clone 2C10A12 (Cat # MAB17913) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with ATG3 monoclonal antibody, clone 2C10A12 (Cat # MAB17913).
Flow Cytometry
Flow cytometric analysis of Jurkat cells with ATG3 monoclonal antibody, clone 2C10A12 (Cat # MAB17913) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human ATG3.
Immunogen
Recombinant protein corresponding to amino acids 1-100 of human ATG3.
Host
Mouse
Theoretical MW (kDa)
35.9
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunofluorescence (1:200-1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: ATG3-hIgGFc transfected HEK293 cell lysates with ATG3 monoclonal antibody, clone 2C10A12 (Cat # MAB17913).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach carcinoma with ATG3 monoclonal antibody, clone 2C10A12 (Cat # MAB17913).Immunofluorescence
Immunofluorescent staining of SMMC-7721 cells with ATG3 monoclonal antibody, clone 2C10A12 (Cat # MAB17913) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis with ATG3 monoclonal antibody, clone 2C10A12 (Cat # MAB17913).Flow Cytometry
Flow cytometric analysis of Jurkat cells with ATG3 monoclonal antibody, clone 2C10A12 (Cat # MAB17913) (Green). Red: Negative Control. -
Gene Info — ATG3
Entrez GeneID
64422Protein Accession#
Q9NT62Gene Name
ATG3
Gene Alias
APG3, APG3-LIKE, APG3L, DKFZp564M1178, FLJ22125, MGC15201, PC3-96
Gene Description
ATG3 autophagy related 3 homolog (S. cerevisiae)
Omim ID
609606Gene Ontology
HyperlinkGene Summary
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM
Other Designations
2610016C12Rik|APG3 autophagy 3-like|Apg3p|autophagy Apg3p/Aut1p-like
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Interactome
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Pathway
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Disease
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