ADAR monoclonal antibody, clone 4E2E4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: ADAR-hIgGFc transfected HEK293 cell lysates with ADAR monoclonal antibody, clone 4E2E4 (Cat # MAB17910).
Immunofluorescence
Immunofluorescent staining of HeLa cells with ADAR monoclonal antibody, clone 4E2E4 (Cat # MAB17910) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with ADAR monoclonal antibody, clone 4E2E4 (Cat # MAB17910).
Flow Cytometry
Flow cytometric analysis of HeLa cells with ADAR monoclonal antibody, clone 4E2E4 (Cat # MAB17910) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human ADAR.
Immunogen
Recombinant protein corresponding to amino acids 1085-1223 of human ADAR.
Host
Mouse
Theoretical MW (kDa)
136
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunofluorescence (1:200-1:1000)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: ADAR-hIgGFc transfected HEK293 cell lysates with ADAR monoclonal antibody, clone 4E2E4 (Cat # MAB17910).Immunofluorescence
Immunofluorescent staining of HeLa cells with ADAR monoclonal antibody, clone 4E2E4 (Cat # MAB17910) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis with ADAR monoclonal antibody, clone 4E2E4 (Cat # MAB17910).Flow Cytometry
Flow cytometric analysis of HeLa cells with ADAR monoclonal antibody, clone 4E2E4 (Cat # MAB17910) (Green). Red: Negative Control. -
Gene Info — ADAR
Entrez GeneID
103Protein Accession#
P55265Gene Name
ADAR
Gene Alias
ADAR1, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88dsRBP, p136
Gene Description
adenosine deaminase, RNA-specific
Gene Ontology
HyperlinkGene Summary
This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
136 kDa double-stranded RNA binding protein|OTTHUMP00000035372|OTTHUMP00000035373|adenosine deaminase acting on RNA 1-A|double-stranded RNA-specific adenosine deaminase|interferon-induced protein 4|interferon-inducible protein 4
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Interactome
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Pathway
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Disease
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