ARFGAP1 monoclonal antibody, clone 1C4E2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: ARFGAP1-hIgGFc transfected HEK293 cell lysates with ARFGAP1 monoclonal antibody, clone 1C4E2 (Cat # MAB17901).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with ARFGAP1 monoclonal antibody, clone 1C4E2 (Cat # MAB17901).
Flow Cytometry
Flow cytometric analysis of HepG2 cells with ARFGAP1 monoclonal antibody, clone 1C4E2 (Cat # MAB17901) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human ARFGAP1.
Immunogen
Recombinant protein corresponding to amino acids 270-414 of human ARFGAP1.
Host
Mouse
Theoretical MW (kDa)
44.7
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: ARFGAP1-hIgGFc transfected HEK293 cell lysates with ARFGAP1 monoclonal antibody, clone 1C4E2 (Cat # MAB17901).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with ARFGAP1 monoclonal antibody, clone 1C4E2 (Cat # MAB17901).Flow Cytometry
Flow cytometric analysis of HepG2 cells with ARFGAP1 monoclonal antibody, clone 1C4E2 (Cat # MAB17901) (Green). Red: Negative Control. -
Gene Info — ARFGAP1
Entrez GeneID
55738Protein Accession#
Q8N6T3Gene Name
ARFGAP1
Gene Alias
ARF1GAP, HRIHFB2281, MGC39924
Gene Description
ADP-ribosylation factor GTPase activating protein 1
Omim ID
608377Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
ADP-ribosylation factor 1 GTPase activating protein|ARF1-directed GTPase-activating protein|GAP protein|OTTHUMP00000031551
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Interactome
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Pathway
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