TUP1 monoclonal antibody, clone 10
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant TUP1.
Immunogen
Recombinant protein corresponding to amino acids 1-200 of Yeast TUP1.
Host
Mouse
Reactivity
Yeast
Form
Liquid
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.08% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of TUP1 expression in Mav108 cells with TUP1 monoclonal antibody, clone 10. -
Gene Info — TUP1
Entrez GeneID
850445Gene Name
TUP1
Gene Alias
AAR1, AER2, AMM1, CRT4, CYC9, FLK1, ROX4, SFL2, UMR7
Gene Description
General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes
Gene Ontology
HyperlinkGene Summary
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Other Designations
Tup1p
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Publication Reference
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Recruitment of O-GlcNAc transferase to promoters by corepressor mSin3A: coupling protein O-GlcNAcylation to transcriptional repression.
Yang X, Zhang F, Kudlow JE.
Cell 2002 Jul; 110(1):69.
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Ssn6-Tup1 interacts with class I histone deacetylases required for repression.
Watson AD, Edmondson DG, Bone JR, Mukai Y, Yu Y, Du W, Stillman DJ, Roth SY.
Genes & Development 2000 Nov; 14(21):2737.
Application:WB, IP, Yeast, Yeast cells.
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Recruitment of the yeast Tup1p-Ssn6p repressor is associated with localized decreases in histone acetylation.
Bone JR, Roth SY.
The Journal of Biological Chemistry 2001 Jan; 276(3):1808.
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Recruitment of O-GlcNAc transferase to promoters by corepressor mSin3A: coupling protein O-GlcNAcylation to transcriptional repression.
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