TFAP2A monoclonal antibody, clone 2H9B5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: TFAP2A-hIgGFc transfected HEK293 cell lysates with TFAP2A monoclonal antibody, clone 2H9B5 (Cat # MAB17892).
Immunofluorescence
Immunofluorescent staining of HeLa cells with TFAP2A monoclonal antibody, clone 2H9B5 (Cat # MAB17892) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with TFAP2A monoclonal antibody, clone 2H9B5 (Cat # MAB17892).
Flow Cytometry
Flow cytometric analysis of HeLa cells with TFAP2A monoclonal antibody, clone 2H9B5 (Cat # MAB17892) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human TFAP2A.
Immunogen
Recombinant protein corresponding to amino acids 105-211 of human TFAP2A.
Host
Mouse
Theoretical MW (kDa)
48
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunofluorescence (1:200-1:1000)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: TFAP2A-hIgGFc transfected HEK293 cell lysates with TFAP2A monoclonal antibody, clone 2H9B5 (Cat # MAB17892).Immunofluorescence
Immunofluorescent staining of HeLa cells with TFAP2A monoclonal antibody, clone 2H9B5 (Cat # MAB17892) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis with TFAP2A monoclonal antibody, clone 2H9B5 (Cat # MAB17892).Flow Cytometry
Flow cytometric analysis of HeLa cells with TFAP2A monoclonal antibody, clone 2H9B5 (Cat # MAB17892) (Green). Red: Negative Control. -
Gene Info — TFAP2A
Entrez GeneID
7020Protein Accession#
P05549Gene Name
TFAP2A
Gene Alias
AP-2, AP-2alpha, AP2TF, BOFS, TFAP2
Gene Description
transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
Omim ID
107580Gene Ontology
HyperlinkGene Summary
AP2-alpha is a 52-kD retinoic acid-inducible and developmentally regulated activator of transcription that binds to a consensus DNA-binding sequence CCCCAGGC in the SV40 and metallothionein (MIM 156350) promoters.[supplied by OMIM
Other Designations
OTTHUMP00000016013|OTTHUMP00000016014|activating enhancer-binding protein 2 alpha|transcription factor AP-2 alpha|transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha)
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Interactome
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Disease
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