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Last updated: 2022/8/14

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STAT5A monoclonal antibody, clone 6D4 

  • Catalog # : MAB17877
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human STAT5A.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 583-794 of human STAT5A.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 90.6
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Western Blot (1:100-1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: K562, Lane 2: MOLT4, Lane 3: HeLa, Lane 4: Jurkat and Lane 5: A431 cell lysates with STAT5A monoclonal antibody, clone 6D4 (Cat # MAB17877).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human ovarian cancer with STAT5A monoclonal antibody, clone 6D4 (Cat # MAB17877).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HeLa cells with STAT5A monoclonal antibody, clone 6D4 (Cat # MAB17877) (Green). Blue: DRAQ5 fluorescent DNA dye.
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis with STAT5A monoclonal antibody, clone 6D4 (Cat # MAB17877).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of K562 cells with STAT5A monoclonal antibody, clone 6D4 (Cat # MAB17877) (Green). Red: Negative Control.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6776
  • Gene Name:
  • STAT5A
  • Gene Alias:
  • MGF,STAT5
  • Gene Description:
  • signal transducer and activator of transcription 5A
  • Gene Summary:
  • The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq
  • Other Designations:
  • -
  • Interactome
  • Interactome
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