NOS2 monoclonal antibody, clone 4E5
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: Jurkat, Lane 2: Jurkat, Lane 3: A549, Lane 4: HeLa, Lane 5: NIH3T3 and Lane 6: MCF-7 cell lysates with NOS2 monoclonal antibody, clone 4E5 (Cat # MAB17874).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with NOS2 monoclonal antibody, clone 4E5 (Cat # MAB17874).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human NOS2.
Immunogen
Recombinant protein corresponding to amino acids 997-1058 of human NOS2.
Host
Mouse
Theoretical MW (kDa)
131
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1/10000)
Flow Cytometry (1/200-1/400)
Immunohistochemistry (1/200-1/1000)
Western Blot (1/500-1/2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% Protein Stabilizer, 0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: Jurkat, Lane 2: Jurkat, Lane 3: A549, Lane 4: HeLa, Lane 5: NIH3T3 and Lane 6: MCF-7 cell lysates with NOS2 monoclonal antibody, clone 4E5 (Cat # MAB17874).Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with NOS2 monoclonal antibody, clone 4E5 (Cat # MAB17874).Flow Cytometry
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Gene Info — NOS2
Entrez GeneID
4843Protein Accession#
P35228Gene Name
NOS2
Gene Alias
HEP-NOS, INOS, NOS, NOS2A
Gene Description
nitric oxide synthase 2, inducible
Gene Ontology
HyperlinkGene Summary
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq
Other Designations
NOS, type II|nitric oxide synthase 2A|nitric oxide synthase 2A (inducible, hepatocytes)|nitric oxide synthase, macrophage
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Interactome
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Pathway
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Disease
- Acute Disease
- Adenocarcinoma
- Albuminuria
- Alzheimer disease
- Anemia
- Angina
- Angina Pectoris
- Arthritis
- Asthma
+ View More Disease
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