AURKA monoclonal antibody, clone 2D8A4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) HEK293, (2) AURKA-hIgGFc transfected HEK293 cell lysate.
Western Blot
Western Blot analysis of (1) HEK293, (2) MCF-7, (3) HeLa cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of rectum cancer tissues with DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of cervical cancer tissues with DAB staining.
Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence
Immunofluorescent staining of SMMC-7721 cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of AURKA monoclonal antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells using AURKA mouse monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human AURKA.
Immunogen
Recombinant protein corresponding to amino acids 268-404 of human AURKA from E. coli.
Host
Mouse
Theoretical MW (kDa)
45.8
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunofluorescence (1:200-1000)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) HEK293, (2) AURKA-hIgGFc transfected HEK293 cell lysate.Western Blot
Western Blot analysis of (1) HEK293, (2) MCF-7, (3) HeLa cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of rectum cancer tissues with DAB staining.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of cervical cancer tissues with DAB staining.Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Immunofluorescence
Immunofluorescent staining of SMMC-7721 cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of AURKA monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using AURKA mouse monoclonal antibody (green) and negative control (red). -
Gene Info — AURKA
Entrez GeneID
6790Gene Name
AURKA
Gene Alias
AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7
Gene Description
aurora kinase A
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
IPL1-related kinase|OTTHUMP00000031340|OTTHUMP00000031342|OTTHUMP00000031343|OTTHUMP00000031344|OTTHUMP00000031345|OTTHUMP00000166071|aurora-A|aurora-related kinase 1|aurora/IPL1-like kinase|breast-tumor-amplified kinase|serine/threonine kinase 15|serine/
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Interactome
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Disease
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