HLA-B monoclonal antibody, clone 2G7B10
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human HLA-B.
Immunogen
Recombinant protein corresponding to amino acids 241-362 of human HLA-B from E. coli.
Host
Mouse
Theoretical MW (kDa)
40.5
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunofluorescence (1:200-1000)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) HEK293, (2) HLA-B-hIgGFc transfected HEK293 cell lysate.Western Blot
Western Blot analysis of (1) Ramos and (2) A431 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of ovarian cancer tissues with DAB staining.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of bladder cancer tissues with DAB staining.Immunofluorescence
Immunofluorescent staining of HeLa cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HLA-B monoclonal antibody.Flow Cytometry
Flow cytometric analysis of MCF-7 cells using HLA-B mouse monoclonal antibody (green) and negative control (red). -
Gene Info — HLA-B
Entrez GeneID
3106Gene Name
HLA-B
Gene Alias
AS, HLA-B-7301, HLA-B73, HLAB, HLAC, SPDA1
Gene Description
major histocompatibility complex, class I, B
Gene Ontology
HyperlinkGene Summary
HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq
Other Designations
HLA class I histocompatibility antigen, B alpha chain|HLA class I histocompatibility antigen, B-7 alpha chain|HLA class I histocompatibility antigen, Cw-5 alpha chain|HLA-B alpha chain (HLA-B*7301)|MHC Class I HLA heavy chain|MHC HLA-B cell surface glycop
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Interactome
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Pathway
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Disease
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