BUB1 monoclonal antibody, clone 1A11C7
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against recombinant human BUB1.
Immunogen
Recombinant protein corresponding to amino acids 1-130 of human BUB1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
122.4
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) HEK293, (2) BUB1-hIgGFc transfected HEK293 cell lysate.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BUB1 monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using BUB1 mouse monoclonal antibody (green) and negative control (red). -
Gene Info — BUB1
Entrez GeneID
699Gene Name
BUB1
Gene Alias
BUB1A, BUB1L, hBUB1
Gene Description
budding uninhibited by benzimidazoles 1 homolog (yeast)
Omim ID
602452Gene Ontology
HyperlinkGene Summary
This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. [provided by RefSeq
Other Designations
BUB1 budding uninhibited by benzimidazoles 1 homolog|budding uninhibited by benzimidazoles 1|mitotic spindle checkpoint kinase|putative serine/threonine-protein kinase
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Interactomes
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Pathways
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Diseases
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