AOF2 monoclonal antibody, clone 3F9D2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) HEK293, (2) AOF2-hIgGFc transfected HEK293 cell lysate.
Western Blot
Western Blot analysis of (1) K562, (2) Jurkat, and (3) HeLa cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of endometrial cancer tissues with DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of esophageal cancer tissues with DAB staining.
Immunofluorescence
Immunofluorescent staining of HL-7702 cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence
Immunofluorescent staining of MCF-7 cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of AOF2 monoclonal antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells using AOF2 mouse monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human AOF2.
Immunogen
Recombinant protein corresponding to amino acids 55-263 of human AOF2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
93
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunofluorescence (1:200-1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) HEK293, (2) AOF2-hIgGFc transfected HEK293 cell lysate.Western Blot
Western Blot analysis of (1) K562, (2) Jurkat, and (3) HeLa cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of endometrial cancer tissues with DAB staining.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of esophageal cancer tissues with DAB staining.Immunofluorescence
Immunofluorescent staining of HL-7702 cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Immunofluorescence
Immunofluorescent staining of MCF-7 cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of AOF2 monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using AOF2 mouse monoclonal antibody (green) and negative control (red). -
Gene Info — AOF2
Entrez GeneID
23028Gene Name
AOF2
Gene Alias
BHC110, KDM1, KIAA0601, LSD1
Gene Description
amine oxidase (flavin containing) domain 2
Omim ID
609132Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
BRAF35-HDAC complex protein BHC110|FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit|lysine (K)-specific demethylase 1|lysine-specific histone demethylase 1
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Interactome
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