TRAF2 monoclonal antibody, clone 5C2C3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) HEK293, (2) TRAF2-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of cervical cancer tissues with DAB staining.
Immunofluorescence
Immunofluorescent staining of MCF-7 cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence
Immunofluorescent staining of HL-7702 cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of TRAF2 monoclonal antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells using TRAF2 mouse monoclonal antibody (green) and negative control (red).
Flow Cytometry
Flow cytometric analysis of HepG2 cells using TRAF2 mouse monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human TRAF2.
Immunogen
Recombinant protein corresponding to amino acids 39-188 of human TRAF2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
55.8
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunofluorescence (1:200-1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1000)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) HEK293, (2) TRAF2-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of cervical cancer tissues with DAB staining.Immunofluorescence
Immunofluorescent staining of MCF-7 cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Immunofluorescence
Immunofluorescent staining of HL-7702 cell line with antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of TRAF2 monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using TRAF2 mouse monoclonal antibody (green) and negative control (red).Flow Cytometry
Flow cytometric analysis of HepG2 cells using TRAF2 mouse monoclonal antibody (green) and negative control (red). -
Gene Info — TRAF2
Entrez GeneID
7186Gene Name
TRAF2
Gene Alias
MGC:45012, TRAP, TRAP3
Gene Description
TNF receptor-associated factor 2
Omim ID
601895Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined. [provided by RefSeq
Other Designations
OTTHUMP00000022625|OTTHUMP00000064745|tumor necrosis factor type 2 receptor associated protein 3
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Interactome
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Pathway
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Disease
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