FOXP3 monoclonal antibody, clone 4F12A2
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human FOXP3.
Immunogen
Recombinant protein corresponding to amino acids 297-431 of human FOXP3 from E. coli.
Host
Mouse
Theoretical MW (kDa)
47.3
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of (1) HEK293, (2) FOXP3-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of ovarian cancer tissues with DAB staining.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of liver cancer tissues with DAB staining.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of FOXP3 monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using FOXP3 mouse monoclonal antibody (green) and negative control (red). -
Gene Info — FOXP3
Entrez GeneID
50943Gene Name
FOXP3
Gene Alias
AIID, DIETER, IPEX, JM2, MGC141961, MGC141963, PIDX, XPID
Gene Description
forkhead box P3
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000025832|immune dysregulation, polyendocrinopathy, enteropathy, X-linked|immunodeficiency, polyendocrinopathy, enteropathy, X-linked|scurfin
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Interactome
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Disease
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