ADIPOQ monoclonal antibody, clone 5B5B10
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ADIPOQ.
Immunogen
Recombinant protein corresponding to amino acids 211-360 of human ADIPOQ from E. coli.
Host
Mouse
Theoretical MW (kDa)
26.4
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:50-1:250)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) MCF-7 cell, (2) HeLa cell, (3) K562 cell, (4) A549 cell, (5) MOLT4 cell with ADIPOQ monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ADIPOQ-hIgGFc transfected HEK293 cell lysate with ADIPOQ monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with ADIPOQ monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with ADIPOQ monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ADIPOQ monoclonal antibody, clone 5B5B10.Flow Cytometry
Flow cytometric analysis of HeLa cells with ADIPOQ monoclonal antibody (green) and negative control (red). -
Gene Info — ADIPOQ
Entrez GeneID
9370Gene Name
ADIPOQ
Gene Alias
ACDC, ACRP30, ADPN, APM-1, APM1, GBP28, adiponectin
Gene Description
adiponectin, C1Q and collagen domain containing
Omim ID
605441Gene Ontology
HyperlinkGene Summary
This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. [provided by RefSeq
Other Designations
adipocyte, C1Q and collagen domain containing|adipocyte, C1Q and collagen domain-containing|adiponectin|adipose most abundant gene transcript 1|gelatin-binding protein 28
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Interactome
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Pathway
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Disease
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