ASH2L monoclonal antibody, clone 5B5B12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) MCF-7 cell, (2) HeLa cell, (3) K562 cell, (4) A549 cell, (5) MOLT4 cell with ASH2L monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ASH2L-hIgGFc transfected HEK293 cell lysate with ASH2L monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissue with ASH2L monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with ASH2L monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ASH2L monoclonal antibody, clone 5B5B12.
Flow Cytometry
Flow cytometric analysis of HeLa cells with ASH2L monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ASH2L.
Immunogen
Recombinant protein corresponding to amino acids 211-360 of human ASH2L from E. coli.
Host
Mouse
Theoretical MW (kDa)
68.7
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:50-1:250)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) MCF-7 cell, (2) HeLa cell, (3) K562 cell, (4) A549 cell, (5) MOLT4 cell with ASH2L monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ASH2L-hIgGFc transfected HEK293 cell lysate with ASH2L monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissue with ASH2L monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with ASH2L monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ASH2L monoclonal antibody, clone 5B5B12.Flow Cytometry
Flow cytometric analysis of HeLa cells with ASH2L monoclonal antibody (green) and negative control (red). -
Gene Info — ASH2L
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Interactome
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+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
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