IGHA1 monoclonal antibody, clone 7D5F12
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human IGHA1.
Immunogen
Recombinant protein corresponding to amino acids 207-353 of human IGHA1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
37.6
Reactivity
Human, Monkey, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:50-1:250)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) L1210 cell, (2) THP-1 cell, (3) HepG2 cell, (4) COS7 cell with IGHA1 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) IGHA1-hIgGFc transfected HEK293 cell lysate with IGHA1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded breast cancer tissue with IGHA1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with IGHA1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of IGHA1 monoclonal antibody, clone 7D5F12.Flow Cytometry
Flow cytometric analysis of HeLa cells with IGHA1 monoclonal antibody (green) and negative control (red). -
Gene Info — IGHA1
Entrez GeneID
3493Gene Name
IGHA1
Gene Alias
FLJ14473, FLJ35065, FLJ35500, FLJ36402, FLJ39698, FLJ40001, FLJ41548, FLJ41552, FLJ41789, FLJ43248, FLJ43594, FLJ44293, FLJ46028, FLJ46621, FLJ46724, FLJ46811, FLJ46824, IgA1, MGC102857
Gene Description
immunoglobulin heavy constant alpha 1
Omim ID
146900Gene Ontology
HyperlinkOther Designations
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Interactome
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Disease
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