EIF5 monoclonal antibody, clone 6D6H11
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human EIF5.
Immunogen
Recombinant protein corresponding to amino acids 1-300 of human EIF5 from E. coli.
Host
Mouse
Theoretical MW (kDa)
49.2
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:50-1:250)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) EIF5-hIgGFc transfected HEK293 cell lysate with EIF5 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissue with EIF5 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with EIF5 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EIF5 monoclonal antibody, clone 6D6H11.Flow Cytometry
Flow cytometric analysis of HeLa cells with EIF5 monoclonal antibody (green) and negative control (red). -
Gene Info — EIF5
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Interactome
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