CSPG4 monoclonal antibody, clone 7G4E5
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CSPG4.
Immunogen
Recombinant protein corresponding to amino acids 2247-2308 of human CSPG4 from E. coli.
Host
Mouse
Theoretical MW (kDa)
250
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry
Immunohistochemistry
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) CSPG4-hIgGFc transfected HEK293 cell lysate with CSPG4 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CSPG4 monoclonal antibody, clone 7G4E5.Flow Cytometry
Flow cytometric analysis of HEK293 cells with CSPG4 monoclonal antibody (green) and negative control (red). -
Gene Info — CSPG4
Entrez GeneID
1464Gene Name
CSPG4
Gene Alias
HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2
Gene Description
chondroitin sulfate proteoglycan 4
Omim ID
601172Gene Ontology
HyperlinkGene Summary
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq
Other Designations
chondroitin sulfate proteoglycan 4 (melanoma-associated)|melanoma-associated chondroitin sulfate proteoglycan
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Interactome
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Disease
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