CLGN monoclonal antibody, clone 9C8G10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) LNCaP cell, (2) HepG2 cell, (3) PC-3 cell, (4) Raji cell with CLGN monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) CLGN-hIgGFc transfected HEK293 cell lysate with CLGN monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded breast cancer tissue with CLGN monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HepG2 cells with CLGN monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CLGN monoclonal antibody, clone 9C8G10.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CLGN.
Immunogen
Recombinant protein corresponding to amino acids 249-405 of human CLGN from E. coli.
Host
Mouse
Theoretical MW (kDa)
70
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry
Immunocytochemistry (1:75)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) LNCaP cell, (2) HepG2 cell, (3) PC-3 cell, (4) Raji cell with CLGN monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) CLGN-hIgGFc transfected HEK293 cell lysate with CLGN monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded breast cancer tissue with CLGN monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HepG2 cells with CLGN monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CLGN monoclonal antibody, clone 9C8G10. -
Gene Info — CLGN
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Interactome
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Disease
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