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Last updated: 2020/6/28

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CLGN monoclonal antibody, clone 9C8G10 

  • Catalog # : MAB17809
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant human CLGN.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 249-405 of human CLGN from E. coli.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 70
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunocytochemistry (1:150)
    Immunohistochemistry (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of (1) LNCaP cell, (2) HepG2 cell, (3) PC-3 cell, (4) Raji cell with CLGN monoclonal antibody.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of (1) HEK293 cells, (2) CLGN-hIgGFc transfected HEK293 cell lysate with CLGN monoclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded breast cancer tissue with CLGN monoclonal antibody.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemical staining of HepG2 cells with CLGN monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis of CLGN monoclonal antibody, clone 9C8G10.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HEK293 cells with CLGN monoclonal antibody (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1047
  • Gene Name:
  • CLGN
  • Gene Alias:
  • -
  • Gene Description:
  • calmegin
  • Gene Summary:
  • Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility. [provided by RefSeq
  • Other Designations:
  • -
  • Related Disease
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