GUCY1A3 monoclonal antibody, clone 3G6B2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) HEK293 cell, (2) Raji cell with GUCY1A3 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissue with GUCY1A3 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HepG2 cells with GUCY1A3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of GUCY1A3 monoclonal antibody, clone 3G6B2.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human GUCY1A3.
Immunogen
Recombinant protein corresponding to amino acids of human 22-214 GUCY1A3 from E. coli.
Host
Mouse
Theoretical MW (kDa)
77.5
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:200-1:1000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HEK293 cell, (2) Raji cell with GUCY1A3 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissue with GUCY1A3 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HepG2 cells with GUCY1A3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of GUCY1A3 monoclonal antibody, clone 3G6B2. -
Gene Info — GUCY1A3
Entrez GeneID
2982Gene Name
GUCY1A3
Gene Alias
GC-SA3, GUC1A3, GUCA3, GUCSA3, GUCY1A1
Gene Description
guanylate cyclase 1, soluble, alpha 3
Omim ID
139396Gene Ontology
HyperlinkGene Summary
Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha subunit, such as alpha-1 (GUCY1A3), and a beta subunit, typically beta-1 (GUCY1B3; MIM 139397), catalyzes conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs (Zabel et al., 1998 [PubMed 9742212]).[supplied by OMIM
Other Designations
GC-S-alpha-1|soluble guanylate cyclase large subunit
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Interactome
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Pathway
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