ID2 monoclonal antibody, clone 4E12G5
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded breast cancer tissue with ID2 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ID2 monoclonal antibody, clone 4E12G5.
Flow Cytometry
Flow cytometric analysis of SK-N-SH cells with ID2 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ID2.
Immunogen
Recombinant protein corresponding to amino acids 1-134 of human ID2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
15
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded breast cancer tissue with ID2 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ID2 monoclonal antibody, clone 4E12G5.Flow Cytometry
Flow cytometric analysis of SK-N-SH cells with ID2 monoclonal antibody (green) and negative control (red). -
Gene Info — ID2
Entrez GeneID
3398Gene Name
ID2
Gene Alias
GIG8, ID2A, ID2H, MGC26389, bHLHb26
Gene Description
inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
Omim ID
600386Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene. [provided by RefSeq
Other Designations
DNA-binding protein inhibitor ID2|OTTHUMP00000140258|cell growth-inhibiting gene 8|helix-loop-helix protein ID2|inhibitor of DNA binding 2|inhibitor of differentiation 2
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Interactome
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Pathway
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Disease
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