CDH5 monoclonal antibody, clone 3D5C7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) MCF-7 cell, (2) A549 cell, (3) HUVE-12 cell, (4) rat lung tissue with CDH5 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CDH5 monoclonal antibody, clone 3D5C7.
Flow Cytometry
Flow cytometric analysis of Jurkat cells with CDH5 monoclonal antibody (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CDH5.
Immunogen
Recombinant protein corresponding to amino acids 29-223 of human CDH5 from E. coli.
Host
Mouse
Theoretical MW (kDa)
87.5
Reactivity
Human, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry
Immunohistochemistry
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) MCF-7 cell, (2) A549 cell, (3) HUVE-12 cell, (4) rat lung tissue with CDH5 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CDH5 monoclonal antibody, clone 3D5C7.Flow Cytometry
Flow cytometric analysis of Jurkat cells with CDH5 monoclonal antibody (green) and negative control (purple). -
Gene Info — CDH5
Entrez GeneID
1003Gene Name
CDH5
Gene Alias
7B4, CD144, FLJ17376
Gene Description
cadherin 5, type 2 (vascular endothelium)
Omim ID
601120Gene Ontology
HyperlinkGene Summary
This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq
Other Designations
7B4 antigen|VE-cadherin|cadherin 5, type 2|cadherin 5, type 2, VE-cadherin (vascular epithelium)|cd144 antigen|endothelial-specific cadherin|vascular endothelial cadherin
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Interactome
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Pathway
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Disease
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