BDNF monoclonal antibody, clone 3D9C5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of SK-N-SH cell with BDNF monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) BDNF-hIgGFc transfected HEK293 cell lysate with BDNF monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with BDNF monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BDNF monoclonal antibody, clone 3D9C5.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human BDNF.
Immunogen
Recombinant protein corresponding to amino acids 19-248 of human BDNF from E. coli.
Host
Mouse
Theoretical MW (kDa)
27.8
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry
Immunocytochemistry (1:200-1:1000)
Immunohistochemistry
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of SK-N-SH cell with BDNF monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) BDNF-hIgGFc transfected HEK293 cell lysate with BDNF monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with BDNF monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BDNF monoclonal antibody, clone 3D9C5. -
Gene Info — BDNF
Entrez GeneID
627Gene Name
BDNF
Gene Alias
MGC34632
Gene Description
brain-derived neurotrophic factor
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq
Other Designations
neurotrophin
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Interactome
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Pathway
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Disease
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