NBN monoclonal antibody, clone 7E4C2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Jurkat cell with NBN monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) NBN-hIgGFc transfected HEK293 cell lysate with NBN monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with NBN monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with NBN monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of NBN monoclonal antibody, clone 7E4C2.
Flow Cytometry
Flow cytometric analysis of HeLa cells with NBN monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human NBN.
Immunogen
Recombinant protein corresponding to amino acids 467-615 of human NBN from E. coli.
Host
Mouse
Theoretical MW (kDa)
85
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:200-1:1000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Jurkat cell with NBN monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) NBN-hIgGFc transfected HEK293 cell lysate with NBN monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with NBN monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with NBN monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of NBN monoclonal antibody, clone 7E4C2.Flow Cytometry
Flow cytometric analysis of HeLa cells with NBN monoclonal antibody (green) and negative control (red). -
Gene Info — NBN
Entrez GeneID
4683Gene Name
NBN
Gene Alias
AT-V1, AT-V2, ATV, FLJ10155, MGC87362, NBS, NBS1, P95
Gene Description
nibrin
Gene Ontology
HyperlinkGene Summary
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq
Other Designations
Nijmegen breakage syndrome 1 (nibrin)|cell cycle regulatory protein p95|p95 protein of the MRE11/RAD50 complex
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Interactome
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Pathway
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Disease
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