EIF2A monoclonal antibody, clone 3A7A8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) MCF-7 cell, (2) PC-12 cell, (3) HepG2 cell, (4) HeLa cell, (5) Cos7 cell, (6) K562 cell, (7) Jurkat cell, (8) A431 cell, (9) NIH/3T3 cell with EIF2A monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) EIF2A-hIgGFc transfected HEK293 cell lysate with EIF2A monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with EIF2A monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HepG2 cells with EIF2A monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EIF2A monoclonal antibody, clone 3A7A8.
Flow Cytometry
Flow cytometric analysis of HepG2 cells with EIF2A monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human EIF2A.
Immunogen
Recombinant protein corresponding to amino acids 448-576 of human EIF2A from E. coli.
Host
Mouse
Theoretical MW (kDa)
65
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:200-1:1000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) MCF-7 cell, (2) PC-12 cell, (3) HepG2 cell, (4) HeLa cell, (5) Cos7 cell, (6) K562 cell, (7) Jurkat cell, (8) A431 cell, (9) NIH/3T3 cell with EIF2A monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) EIF2A-hIgGFc transfected HEK293 cell lysate with EIF2A monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with EIF2A monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HepG2 cells with EIF2A monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of EIF2A monoclonal antibody, clone 3A7A8.Flow Cytometry
Flow cytometric analysis of HepG2 cells with EIF2A monoclonal antibody (green) and negative control (red). -
Gene Info — EIF2A
Entrez GeneID
83939Gene Name
EIF2A
Gene Alias
CDA02, EIF-2A, MST089, MSTP004, MSTP089
Gene Description
eukaryotic translation initiation factor 2A, 65kDa
Omim ID
609234Gene Ontology
HyperlinkGene Summary
EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).[supplied by OMIM
Other Designations
eukaryotic translation initiation factor 2A
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Interactome
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Disease
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