PPP1R1B monoclonal antibody, clone 2G6D9
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) PPP1R1B-hIgGFc transfected HEK293 cell lysate with PPP1R1B monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PPP1R1B monoclonal antibody, clone 2G6D9.
Flow Cytometry
Flow cytometric analysis of HeLa cells with PPP1R1B monoclonal antibody (green) and negative control (red).
-
Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PPP1R1B.
Immunogen
Recombinant protein corresponding to amino acids 95-204 of human PPP1R1B from E. coli.
Host
Mouse
Theoretical MW (kDa)
23
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry
Immunohistochemistry
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) PPP1R1B-hIgGFc transfected HEK293 cell lysate with PPP1R1B monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PPP1R1B monoclonal antibody, clone 2G6D9.Flow Cytometry
Flow cytometric analysis of HeLa cells with PPP1R1B monoclonal antibody (green) and negative control (red). -
Gene Info — PPP1R1B
Entrez GeneID
84152Gene Name
PPP1R1B
Gene Alias
DARPP-32, DARPP32, FLJ20940
Gene Description
protein phosphatase 1, regulatory (inhibitor) subunit 1B
Omim ID
604399Gene Ontology
HyperlinkGene Summary
Midbrain dopaminergic neurons play a critical role in multiple brain functions, and abnormal signaling through dopaminergic pathways has been implicated in several major neurologic and psychiatric disorders. One well-studied target for the actions of dopamine is DARPP32. In the densely dopamine- and glutamate-innervated rat caudate-putamen, DARPP32 is expressed in medium-sized spiny neurons (Ouimet and Greengard, 1990 [PubMed 2191086]) that also express dopamine D1 receptors (Walaas and Greengard, 1984 [PubMed 6319627]). The function of DARPP32 seems to be regulated by receptor stimulation. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions (Halpain et al., 1990 [PubMed 2153935]). Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32 (Walaas and Greengard, 1984 [PubMed 6319627]); phosphorylated DARPP32 is a potent protein phosphatase-1 (see MIM 176875) inhibitor (Hemmings et al., 1984 [PubMed 6087160]). NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32 (Halpain et al., 1990 [PubMed 2153935]).[supplied by OMIM
Other Designations
OTTHUMP00000164276|dopamine and cAMP regulated phosphoprotein|dopamine and cAMP-regulated neuronal phosphoprotein 32|protein phosphatase 1, regulatory (inhibitor) subunit 1B (dopamine and cAMP regulated phosphoprotein, DARPP-32)
-
Interactome
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com