RPTOR monoclonal antibody, clone 6G9C4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) RPTOR-hIgGFc transfected HEK293 cell lysate with RPTOR monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RPTOR monoclonal antibody, clone 6G9C4.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human RPTOR.
Immunogen
Recombinant protein corresponding to amino acids 874-1009 of human RPTOR from E. coli.
Host
Mouse
Theoretical MW (kDa)
149
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry
Immunocytochemistry
Immunohistochemistry
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) RPTOR-hIgGFc transfected HEK293 cell lysate with RPTOR monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RPTOR monoclonal antibody, clone 6G9C4. -
Gene Info — RPTOR
Entrez GeneID
57521Gene Name
RPTOR
Gene Alias
KOG1, Mip1
Gene Description
regulatory associated protein of MTOR, complex 1
Omim ID
607130Gene Ontology
HyperlinkGene Summary
This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats|regulatory associated protein of mTOR
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Interactome
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Pathway
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Disease
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