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Last updated: 2019/12/8

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DNM1L monoclonal antibody, clone 4A4C7 

  • Catalog # : MAB17781
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant human DNM1L.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 69-213 of human DNM1L from E. coli.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 81.9
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunocytochemistry (1:200-1:1000)
    Immunohistochemistry
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of (1) PC-12 cell, (2) LNCAP cell, (3) NIH/3T3 cell with DNM1L monoclonal antibody.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of (1) HEK293 cells, (2) DNM1L-hIgGFc transfected HEK293 cell lysate with DNM1L monoclonal antibody.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemical staining of HeLa cells with DNM1L monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis of DNM1L monoclonal antibody, clone 4A4C7.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HEK293 cells with DNM1L monoclonal antibody (green) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Gene Name:
  • DNM1L
  • Gene Alias:
  • DLP1,DRP1,DVLP,DYMPLE,DYNIV-11,FLJ41912,HDYNIV,VPS1
  • Gene Description:
  • dynamin 1-like
  • Gene Summary:
  • The protein encoded by this gene is a member of the dynamin superfamily of GTPases. Members of the dynamin-related subfamily, including the S. cerevisiae proteins Dnm1 and Vps1, contain the N-terminal tripartite GTPase domain but do not have the pleckstrin homology or proline-rich domains. This protein establishes mitochondrial morphology through a role in distributing mitochondrial tubules throughout the cytoplasm. The gene has 3 alternatively spliced transcripts encoding different isoforms. These transcripts are alternatively polyadenylated. [provided by RefSeq
  • Other Designations:
  • Dnm1p/Vps1p-like protein,dynamin 1-like protein,dynamin family member proline-rich carboxyl-terminal domain less,dynamin-like protein 4,dynamin-like protein IV,dynamin-related protein 1
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