DNM1L monoclonal antibody, clone 4A4C7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) PC-12 cell, (2) LNCAP cell, (3) NIH/3T3 cell with DNM1L monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) DNM1L-hIgGFc transfected HEK293 cell lysate with DNM1L monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with DNM1L monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DNM1L monoclonal antibody, clone 4A4C7.
Flow Cytometry
Flow cytometric analysis of HEK293 cells with DNM1L monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human DNM1L.
Immunogen
Recombinant protein corresponding to amino acids 69-213 of human DNM1L from E. coli.
Host
Mouse
Theoretical MW (kDa)
81.9
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:200-1:1000)
Immunohistochemistry
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) PC-12 cell, (2) LNCAP cell, (3) NIH/3T3 cell with DNM1L monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) DNM1L-hIgGFc transfected HEK293 cell lysate with DNM1L monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with DNM1L monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DNM1L monoclonal antibody, clone 4A4C7.Flow Cytometry
Flow cytometric analysis of HEK293 cells with DNM1L monoclonal antibody (green) and negative control (red). -
Gene Info — DNM1L
Entrez GeneID
10059Gene Name
DNM1L
Gene Alias
DLP1, DRP1, DVLP, DYMPLE, DYNIV-11, FLJ41912, HDYNIV, VPS1
Gene Description
dynamin 1-like
Omim ID
603850Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dynamin superfamily of GTPases. Members of the dynamin-related subfamily, including the S. cerevisiae proteins Dnm1 and Vps1, contain the N-terminal tripartite GTPase domain but do not have the pleckstrin homology or proline-rich domains. This protein establishes mitochondrial morphology through a role in distributing mitochondrial tubules throughout the cytoplasm. The gene has 3 alternatively spliced transcripts encoding different isoforms. These transcripts are alternatively polyadenylated. [provided by RefSeq
Other Designations
Dnm1p/Vps1p-like protein|dynamin 1-like protein|dynamin family member proline-rich carboxyl-terminal domain less|dynamin-like protein 4|dynamin-like protein IV|dynamin-related protein 1
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Interactome
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Pathway
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Disease
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