ZEB1 monoclonal antibody, clone 2A8H3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ZEB1-hIgGFc transfected HEK293 cell lysate with ZEB1 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with ZEB1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with ZEB1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ZEB1 monoclonal antibody, clone 2A8H3.
Flow Cytometry
Flow cytometric analysis of HeLa cells with ZEB1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ZEB1.
Immunogen
Recombinant protein corresponding to amino acids 967-1108 of human ZEB1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
124
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:200-1:1000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ZEB1-hIgGFc transfected HEK293 cell lysate with ZEB1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with ZEB1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with ZEB1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ZEB1 monoclonal antibody, clone 2A8H3.Flow Cytometry
Flow cytometric analysis of HeLa cells with ZEB1 monoclonal antibody (green) and negative control (red). -
Gene Info — ZEB1
Entrez GeneID
6935Gene Name
ZEB1
Gene Alias
AREB6, BZP, DELTA-EF1, MGC133261, NIL-2-A, NIL-2A, NIL2A, TCF8, ZEB, ZFHEP, ZFHX1A
Gene Description
zinc finger E-box binding homeobox 1
Gene Ontology
HyperlinkGene Summary
ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM
Other Designations
OTTHUMP00000019405|transcription factor 8 (represses interleukin 2 expression)|zinc finger homeodomain enhancer-binding protein
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Interactome
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Disease
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