ABCC4 monoclonal antibody, clone 2D2A9
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ABCC4.
Immunogen
Recombinant protein corresponding to amino acids 631-692 of human ABCC4 from E. coli.
Host
Mouse
Theoretical MW (kDa)
150
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ABCC4-hIgGFc transfected HEK293 cell lysate with ABCC4 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissue with ABCC4 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ABCC4 monoclonal antibody, clone 2D2A9.Flow Cytometry
Flow cytometric analysis of A549 cells with ABCC4 monoclonal antibody (green) and negative control (red). -
Gene Info — ABCC4
Entrez GeneID
10257Gene Name
ABCC4
Gene Alias
EST170205, MOAT-B, MOATB, MRP4
Gene Description
ATP-binding cassette, sub-family C (CFTR/MRP), member 4
Omim ID
605250Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein has not yet been determined; however, this protein may play a role in cellular detoxification as a pump for its substrate, organic anions. Alternative splicing results in multiple splice variants encoding different isoforms. [provided by RefSeq
Other Designations
ATP-binding cassette, sub-family C, member 4|OTTHUMP00000018560|bA464I2.1 (ATP-binding cassette, sub-family C (CFTR/MRP), member 4)|canalicular multispecific organic anion transporter (ABC superfamily)|multidrug resistance-associated protein 4|multispecif
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