GPNMB monoclonal antibody, clone 7C10E5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) PANC1 cell, (2) PC-3 cell with GPNMB monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) GPNMB-hIgGFc transfected HEK293 cell lysate with GPNMB monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded breast cancer tissue with GPNMB monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of GPNMB monoclonal antibody, clone 7C10E5.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human GPNMB.
Immunogen
Recombinant protein corresponding to amino acids 31-260 of human GPNMB from E. coli.
Host
Mouse
Theoretical MW (kDa)
64
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) PANC1 cell, (2) PC-3 cell with GPNMB monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) GPNMB-hIgGFc transfected HEK293 cell lysate with GPNMB monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded breast cancer tissue with GPNMB monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of GPNMB monoclonal antibody, clone 7C10E5. -
Gene Info — GPNMB
Entrez GeneID
10457Gene Name
GPNMB
Gene Alias
HGFIN, NMB
Gene Description
glycoprotein (transmembrane) nmb
Omim ID
604368Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
glycoprotein NMB|glycoprotein nmb-like protein|osteoactivin|transmembrane glycoprotein
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Interactome
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Disease
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