IL3RA monoclonal antibody, clone 8E11C5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) IL3RA-hIgGFc transfected HEK293 cell lysate with IL3RA monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded lung cancer tissue with IL3RA monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with IL3RA monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of IL3RA monoclonal antibody, clone 8E11C5.
Flow Cytometry
Flow cytometric analysis of HeLa cells with IL3RA monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human IL3RA.
Immunogen
Recombinant protein corresponding to amino acids 200-305 of human IL3RA from E. coli.
Host
Mouse
Theoretical MW (kDa)
43.3
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:200-1:1000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) IL3RA-hIgGFc transfected HEK293 cell lysate with IL3RA monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded lung cancer tissue with IL3RA monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with IL3RA monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of IL3RA monoclonal antibody, clone 8E11C5.Flow Cytometry
Flow cytometric analysis of HeLa cells with IL3RA monoclonal antibody (green) and negative control (red). -
Gene Info — IL3RA
Entrez GeneID
3563Gene Name
IL3RA
Gene Alias
CD123, IL3R, IL3RAY, IL3RX, IL3RY, MGC34174, hIL-3Ra
Gene Description
interleukin 3 receptor, alpha (low affinity)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. [provided by RefSeq
Other Designations
CD123 antigen|Interleukin-3 receptor|OTTHUMP00000022828|interleukin 3 receptor, alpha
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Interactome
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Pathway
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Disease
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