NRCAM monoclonal antibody, clone 7D8C5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) NRCAM-hIgGFc transfected HEK293 cell lysate with NRCAM monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HepG2 cells with NRCAM monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of NRCAM monoclonal antibody, clone 7D8C5.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human NRCAM.
Immunogen
Recombinant protein corresponding to amino acids 1192-1255 of human NRCAM from E. coli.
Host
Mouse
Theoretical MW (kDa)
144
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry
Immunocytochemistry (1:50)
Immunohistochemistry
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) NRCAM-hIgGFc transfected HEK293 cell lysate with NRCAM monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HepG2 cells with NRCAM monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of NRCAM monoclonal antibody, clone 7D8C5. -
Gene Info — NRCAM
Entrez GeneID
4897Gene Name
NRCAM
Gene Alias
KIAA0343, MGC138845, MGC138846
Gene Description
neuronal cell adhesion molecule
Omim ID
601581Gene Ontology
HyperlinkGene Summary
Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
NgCAM-related cell adhesion molecule|NrCAM-|bravo
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Interactome
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Pathway
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Disease
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