HAS2 monoclonal antibody, clone 4E7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) NTERA-2 cell, (2) HEK293 cell with HAS2 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissue with HAS2 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with HAS2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HAS2 monoclonal antibody, clone 4E7.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human HAS2.
Immunogen
Recombinant protein corresponding to amino acids 67-170 of human HAS2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
63.5
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry
Immunocytochemistry (1:100-1:500)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) NTERA-2 cell, (2) HEK293 cell with HAS2 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissue with HAS2 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with HAS2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HAS2 monoclonal antibody, clone 4E7. -
Gene Info — HAS2
Entrez GeneID
3037Gene Name
HAS2
Gene Alias
MGC126241, MGC126242
Gene Description
hyaluronan synthase 2
Omim ID
601636Gene Ontology
HyperlinkGene Summary
Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1. [provided by RefSeq
Other Designations
hyaluronate synthase 2|hyaluronic acid synthase 2
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Interactome
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Disease
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