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Last updated: 2021/6/20
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ILK monoclonal antibody, clone 3A9 

  • Catalog # : MAB17745
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant human ILK.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 97-244 of human ILK from E. coli.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 51.4
  • Reactivity:
  • Human, Monkey, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunocytochemistry
    Immunohistochemistry (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of (1) Jurkat cell, (2) NIH/3T3, (3) HeLa cell, (4) PC-12 cell, (5) C6 cell, (6) COS7 cell, (7) Raji cell, (8) K562 cell, (9) MCF-7 cell with ILK monoclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded esophageal cancer tissue with ILK monoclonal antibody.
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis of ILK monoclonal antibody, clone 3A9.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Jurkat cells with ILK monoclonal antibody (green) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3611
  • Gene Name:
  • ILK
  • Gene Alias:
  • DKFZp686F1765,P59
  • Gene Description:
  • integrin-linked kinase
  • Gene Summary:
  • Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
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