ILK monoclonal antibody, clone 3A9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) Jurkat cell, (2) NIH/3T3, (3) HeLa cell, (4) PC-12 cell, (5) C6 cell, (6) COS7 cell, (7) Raji cell, (8) K562 cell, (9) MCF-7 cell with ILK monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissue with ILK monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ILK monoclonal antibody, clone 3A9.
Flow Cytometry
Flow cytometric analysis of Jurkat cells with ILK monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ILK.
Immunogen
Recombinant protein corresponding to amino acids 97-244 of human ILK from E. coli.
Host
Mouse
Theoretical MW (kDa)
51.4
Reactivity
Human, Monkey, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) Jurkat cell, (2) NIH/3T3, (3) HeLa cell, (4) PC-12 cell, (5) C6 cell, (6) COS7 cell, (7) Raji cell, (8) K562 cell, (9) MCF-7 cell with ILK monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissue with ILK monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ILK monoclonal antibody, clone 3A9.Flow Cytometry
Flow cytometric analysis of Jurkat cells with ILK monoclonal antibody (green) and negative control (red). -
Gene Info — ILK
Entrez GeneID
3611Gene Name
ILK
Gene Alias
DKFZp686F1765, P59
Gene Description
integrin-linked kinase
Omim ID
602366Gene Ontology
HyperlinkGene Summary
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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