FZD5 monoclonal antibody, clone 2D12
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human FZD5.
Immunogen
Recombinant protein corresponding to amino acids 151-217 of human FZD5 from E. coli.
Host
Mouse
Theoretical MW (kDa)
64.5
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry
Immunohistochemistry
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) A549 cell, (2) PC-3 cell with FZD5 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of FZD5 monoclonal antibody, clone 2D12.Flow Cytometry
Flow cytometric analysis of HepG2 cells with FZD5 monoclonal antibody (green) and negative control (red). -
Gene Info — FZD5
Entrez GeneID
7855Gene Name
FZD5
Gene Alias
C2orf31, DKFZp434E2135, HFZ5, MGC129692
Gene Description
frizzled homolog 5 (Drosophila)
Omim ID
601723Gene Ontology
HyperlinkGene Summary
Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand. [provided by RefSeq
Other Designations
Wnt receptor|frizzled 5|seven-transmembrane receptor frizzled-5
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Interactome
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