SPIB monoclonal antibody, clone 4G5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) A549 cell, (2) PC-3 cell, (3) NIH/3T3 cell with SPIB monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissue with SPIB monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with SPIB monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SPIB monoclonal antibody, clone 4G5.
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells with SPIB monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human SPIB.
Immunogen
Recombinant protein corresponding to amino acids 200-252 of human SPIB from E. coli.
Host
Mouse
Theoretical MW (kDa)
28.8
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:200-1:1000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) A549 cell, (2) PC-3 cell, (3) NIH/3T3 cell with SPIB monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissue with SPIB monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with SPIB monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SPIB monoclonal antibody, clone 4G5.Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells with SPIB monoclonal antibody (green) and negative control (red). -
Gene Info — SPIB
Entrez GeneID
6689Gene Name
SPIB
Gene Alias
SPI-B
Gene Description
Spi-B transcription factor (Spi-1/PU.1 related)
Omim ID
606802Gene Ontology
HyperlinkGene Summary
SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1; MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGGAA-3-prime).[supplied by OMIM
Other Designations
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Interactome
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