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Last updated: 2019/12/1
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SPIB monoclonal antibody, clone 4G5 

  • Catalog # : MAB17740
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant human SPIB.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 200-252 of human SPIB from E. coli.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 28.8
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunocytochemistry (1:200-1:1000)
    Immunohistochemistry (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of (1) A549 cell, (2) PC-3 cell, (3) NIH/3T3 cell with SPIB monoclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded ovarian cancer tissue with SPIB monoclonal antibody.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemical staining of HeLa cells with SPIB monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis of SPIB monoclonal antibody, clone 4G5.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of NIH/3T3 cells with SPIB monoclonal antibody (green) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6689
  • Gene Name:
  • SPIB
  • Gene Alias:
  • SPI-B
  • Gene Description:
  • Spi-B transcription factor (Spi-1/PU.1 related)
  • Gene Summary:
  • SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1; MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGGAA-3-prime).[supplied by OMIM
  • Other Designations:
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