POU3F2 monoclonal antibody, clone 6H12
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human POU3F2.
Immunogen
Recombinant protein corresponding to amino acids 214-362 of human POU3F2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
46.9
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry
Immunocytochemistry
Immunohistochemistry
Western Blot (1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HeLa cell, (2) SK-N-SH cell with POU3F2 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of POU3F2 monoclonal antibody, clone 6H12. -
Gene Info — POU3F2
Entrez GeneID
5454Gene Name
POU3F2
Gene Alias
BRN2, OCT7, OTF7, POUF3
Gene Description
POU class 3 homeobox 2
Omim ID
600494Gene Ontology
HyperlinkGene Summary
POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM
Other Designations
POU domain, class 3, transcription factor 2
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