SRY monoclonal antibody, clone 1G4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of NTERA-2 cell with SRY monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with SRY monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SRY monoclonal antibody, clone 1G4.
Flow Cytometry
Flow cytometric analysis of HepG2 cells with SRY monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human SRY.
Immunogen
Recombinant protein corresponding to amino acids 1-204 of human SRY from E. coli.
Host
Mouse
Theoretical MW (kDa)
23.9
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NTERA-2 cell with SRY monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with SRY monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SRY monoclonal antibody, clone 1G4.Flow Cytometry
Flow cytometric analysis of HepG2 cells with SRY monoclonal antibody (green) and negative control (red). -
Gene Info — SRY
Entrez GeneID
6736Gene Name
SRY
Gene Alias
TDF, TDY
Gene Description
sex determining region Y
Omim ID
480000Gene Ontology
HyperlinkGene Summary
This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome. [provided by RefSeq
Other Designations
OTTHUMP00000034324|essential protein for sex determination in human males|sex determining region protein|sex-determining region on Y|testis-determining factor
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Interactome
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Disease
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