NR6A1 monoclonal antibody, clone 4G8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) K562 cell, (2) NTERA-2 cell, (3) HEK293 cell, (4) HUVE-12 cell, (5) HeLa cell with NR6A1 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) NR6A1-hIgGFc transfected HEK293 cell lysate with NR6A1 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectum cancer tissue with NR6A1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HepG2 cells with NR6A1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of NR6A1 monoclonal antibody, clone 4G8.
Flow Cytometry
Flow cytometric analysis of K562 cells with NR6A1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human NR6A1.
Immunogen
Recombinant protein corresponding to amino acids 65-118 of human NR6A1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
54.3
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:400)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) K562 cell, (2) NTERA-2 cell, (3) HEK293 cell, (4) HUVE-12 cell, (5) HeLa cell with NR6A1 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) NR6A1-hIgGFc transfected HEK293 cell lysate with NR6A1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectum cancer tissue with NR6A1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HepG2 cells with NR6A1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of NR6A1 monoclonal antibody, clone 4G8.Flow Cytometry
Flow cytometric analysis of K562 cells with NR6A1 monoclonal antibody (green) and negative control (red). -
Gene Info — NR6A1
Entrez GeneID
2649Gene Name
NR6A1
Gene Alias
GCNF, GCNF1, NR61, RTR
Gene Description
nuclear receptor subfamily 6, group A, member 1
Omim ID
602778Gene Ontology
HyperlinkGene Summary
This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. The gene expresses at least alternatively spliced transcript variants. [provided by RefSeq
Other Designations
germ cell nuclear factor|retinoic acid receptor-related testis-associated receptor
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Interactome
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Disease
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Publication Reference
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Let-7a-5p abrogates progression of papillary thyroid carcinoma cells by decreasing nuclear receptor subfamily 6 group a member 1-mediated lipogenesis.
Tao Zhao, Jinghui Sun, Xiangdong Lu, Lingling Liu, Lin Chen, Wei Zhao, Bin Zhou.
Journal of Biochemical and Molecular Toxicology 2023 Oct; 31:e23572.
Application：WB, IHC, Human, BC-PAP cells, TPC1 cells.
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Let-7a-5p abrogates progression of papillary thyroid carcinoma cells by decreasing nuclear receptor subfamily 6 group a member 1-mediated lipogenesis.
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