MELK monoclonal antibody, clone 2G2
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human MELK.
Immunogen
A synthetic peptide corresponding to amino acid 637-651 of human MELK.
Host
Mouse
Theoretical MW (kDa)
74.6
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:200-1:1000)
Immunohistochemistry (1:200-1:1000)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded liver cancer tissue with MELK monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HepG2 cells with MELK monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of MELK monoclonal antibody, clone 2G2. -
Gene Info — MELK
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Interactome
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